A good inflammation resolution is associated with a correct healing and tissue repair. Ambiotis has recently developed new approaches in the area of healing, remodeling and regeneration. Our scratch assay allows the accurate evaluation of compound capacity to accelerate healing. It can be combined with biochemical analysis of lipids or cytokines. Our model of fibroblasts in co-culture with macrophages is perfectly suitable to observe the impact of compounds on the switch from pro-inflammatory to regenerative macrophages, the production of lipids involved in healing process (MCTR) and the remodeling of the extracellular matrix.
The Ambiotis news
Phagocytosis is one of the hallmark of inflammation resolution. Ambiotis has developed an assay to monitor phagocytosis in kinetic using High Content Imaging system and evaluate influence of compounds.
Images are then quantified with our partner IMACTIV 3D, a specialist of image analysis.
How do tissues fight against infection and regenerate?
Inflammation resolution may indeed be involved in these fascinating biological processes through the action of the SPMs (Specialized Pro-resolving Mediators).
In this recent publication, Chiang et al. identified genes and pathways that might link resolution of inflammation and regeneration using planaria model. They showed how tissue regeneration is regulated by cys-SPMs pathways, and in particular by TRAF3. TRAF3 indeed contributes to cys-SPM–stimulated phagocyte functions and resolution of infection.