Expertise of Ambiotis

Ambiotis is a research-based company specialized in well-being and tissue homeostasis. Inflammatory components are described in most skin troubles from irritations up to psoriasis, atopic dermatitis and aging.

Ambiotis has thus developed a broad range of innovative cellular tests to evaluate the efficiency of active ingredients on skin protection against homeostasis rupture, pollution, ageing, irritations, scars, atopic dermatitis, psoriasis…

Innovative concepts for dermo-cosmetic products

Homeostasis protection
Evaluate how your active can protect and maintain an healthy balance of the skin by boosting resolutive molecules
Anti-pollution
Evaluate your active for innovative anti-pollution activity on cells or skin models
Anti-Irritation
Evaluate your active for anti-irritation activity on cells or skin models
Barrier function/Defense
Evaluate how a compound can enhance barrier function against water loss, pathogens…
Anti-ageing
Learn if your active is able to counterbalance bad effects of aging (elasticity loss, low grade inflammation…)
Anti-acne
Evaluate if your active allows to fight against bacteria, associated inflammation or to regulate sebum production
Healing
Evaluate the capacities of actives to correctly end inflammatory events and avoid scars
Pigmentation
Evaluate impact of actives on pigmentation on human melanocytes
Skin diseases
Evaluate inflammatory and immunomodulatory impacts of compounds in relevant human cell models (skin, monocytes, macrophages, lymphocytes, co-culture …)
Slimming
Evaluate impact of actives on lipolysis activity in adipocytes
 

Others

We can also address chronobiology, oxidative stress… Do not hesitate to ask for more informations

How to evaluate actives for skin protection against homeostasis rupture, pollution, aging, skin diseases…?

Inflammation is a natural response of the tissue to irritation or rupture of microbiota balance and proliferation of unwanted microorganisms. If correctly controlled, it leads to resolution and allow the tissue to go back to homeostasis.

Resolution is characterized by the natural and sequential synthesis of specialized mediators (resolvins, protectins, lipoxins, maresins) or SPMs. These mediators are essential to induce correct healing, maintain tissue balance and avoid skin discomfort, scars or even chronic inflammation.

SPMs have known biological and beneficial activities in many inflammatory contexts including aging, atopic dermatitis, psoriasis, tissue healing and soothing.

- Anti-inflammation approach: evaluate inhibitory effects of ingredients on pro- inflammatory mediators

ex : secretion of TNFalpha by keratinocytes under PMA treatment

- Pro-resolution approach: evaluate activating effects of ingredients on resolvin, protectin, maresin and lipoxin production

From screening to efficacy validation

Active screening and positioning

Thanks to an array based on RT-qPCR and the possibility to completely customize the design of experimental conditions and the genes of interest, all ideas can be tested:

- Classify your compound library on their impact on cell differentiation, defence, oxidative stress, inflammation, hydration…

- Screen several actives on a specific activity to find candidate (aging, inflammation, resolution, skin elasticity…)

- Characterize a biological process in kinetic

- Compare different models (different cell types, reconstructed skin, explants…) on specific pathways or activity

- Find new activities for a compound for repositioning purposes

Functional assays to validate actives

Evaluate your actives on their ability to protect skin health in your interest area (pollution, acne …) while specifying:

- Impact on skin compartment (epidermis, dermis)

- Crosstalk between cells within same compartment of from different compartment (fibroblasts/macrophages; Langerhans cells/keratinocytes…)

- Role of immune cells on skin health and beauty

Imaging

Skin applications may be investigated using imaging approaches

In vivo evaluation

Analysis of sebum, skin strips, corneodiscs, skin blisters, skin explants, biopsies…

Cell-based assays: human primary cell models

  • Pre-adipocytes and adipocytes
  • Keratinocytes
  • Fibroblasts
  • Melanocytes
  • Immunity cells (dendritic and Langerhans cells, monocytes, macrophages, neutrophils, lymphocytes)
  • Co-culture to mimic skin compartment
  • Reconstructed human skin
  • Skin explant

Senescence can be chemically induced in skin cells.

Donors can be chosen based on their age and sex.

Analytical services

Lipid analysis

- Skin and sebum lipids : squalene, fatty acids (saturated, mono/poly-unsaturated …), sapienic acid, sebaleic acid, cholesterol, sterols, triglycerides, phospholipids, ceramides

- Eicosanoids (Inflammatory mediators, SPMs)

- Endocannabinoids

Techniques: LC-MS/MS or GC-FID

Cytokine and chemokines

- Inflammatory cytokines and chemokines

- Anti-inflammatory cytokines

Techniques: ELISA, Milliplex, Flow cytometry

Gene expression

Single gene expression

Profiling using microfluidic dynamic array

Techniques: RT-qPCR

Oxidative stress markers

- Reactive oxygen species

Techniques: Luminometry (real-time assays,) Flow Cytometry (accumulation), Fluorometry, Mass spectrometry

Cells and Tissues

Techniques: Flow cytometry, Histology

Ways of working with us

 

Step 1: your need

  • Understanding of your need
  • Orientation toward the relevant work package

- analytical package : provide us with samples to be analyzed

- model-based package : provide us with your compounds to evaluate

- Customized development

- Scientific consulting

Step 2: our collaboration

  • Performance of services according milestones and go/no go steps
  • Regular follow-up

Step 3: your results

  • Scientific feedback through webex conference
  • Final report
  • Follow up